Yes.
I actually have 1/2" horse stall mats on top of 3/4" plywood in my garage. I have a 4' by 4' sheet of 1/2" plywood in between the horse stall mats on the ends, specifically for that harder surface to push on.
I think most would recommend more layers of plywood than I have. I just wanted something easy to takedown if and when I move.